![]() ![]() Purity above 95% may be achieved after this first step, which means that only limited polishing is required before the final formulation steps. In monoclonal antibody production, protein A affinity chromatography is widely used for the capture step. The most efficient way to reduce impurities is to start purification with an affinity step as first capture step, whenever possible. The next step is polishing chromatography, which will help you obtain higher purity of the target molecule by clearing remaining impurities (Fig 1). In the downstream bioprocessing phase of biopharmaceutical manufacturing, the first chromatographic capture step isolates the product from the bulk of impurities, such as cell culture media components and proteases. What is polishing chromatography and when is it used? ![]() This article shares the factors you should consider when developing your polishing steps - from the basics to the typical challenges faced. Polishing chromatography is the term we use to describe the removal of minute amounts of impurities in the final phase of biopharmaceutical manufacturing. ![]() By Tomas Björkman, Senior Scientist, Cytiva ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |